Composite

Part:BBa_K3198007:Design

Designed by: Chunyang Song   Group: iGEM19_NUS_Singapore   (2019-09-27)


HicA-LuxABCDE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 549
    Illegal NheI site found at 1767
    Illegal NheI site found at 4733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3731
    Illegal BamHI site found at 489
    Illegal BamHI site found at 1706
    Illegal XhoI site found at 4561
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6120
    Illegal BsaI.rc site found at 3129
    Illegal SapI site found at 1523
    Illegal SapI.rc site found at 6445


Design Notes

NIL


Source

HicA originated from E. coli K12 and its sequence was synthesized by IDT. LuxABCDE sequence is obtained from iGEM repository.

References